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PhD position (60%) “Maintenance of the nuclear pore complex in aged cells and their plasticity in cancer”

ID: 144801

Art des Jobs Vollzeit
Eingetragen am 12.07.2019
Einsatzort Heidelberg

Jobbeschreibung The nuclear pore complex (NPC) is a large cylindrical structure of over 30 different proteins named nucleoporins (NUPs) that is embedded in the nuclear envelope. It facilitates the essential process of nuclear-cytoplasmic transport of RNA molecules and proteins.

NPCs assemble in the intact nuclear envelope by an inside-out mechanism. How this process works, is modulated during aging and changed in cancer cells is largely unknown and will be studied in this project. We have developed a fast and highly efficient CRISPR approach for gene tagging. With this in hand, we will tag endogenous NUPs with fluorophores such as NeonGreen and degron tags and study their behaviour, function and biogenesis in different cell lines and under various growth conditions.

The PhD student will be using a broad range of techniques such as biochemical approaches, conventional light and super resolution microscopy (STED; SIM) and electron microscopy for the analysis of the NPCs. CRISPR technology will be used for genomic tagging of human NUPs.
Qualifikationen The nuclear pore complex (NPC) is a large cylindrical structure of over 30 different proteins named nucleoporins (NUPs) that is embedded in the nuclear envelope. It facilitates the essential process of nuclear-cytoplasmic transport of RNA molecules and proteins.

NPCs assemble in the intact nuclear envelope by an inside-out mechanism. How this process works, is modulated during aging and changed in cancer cells is largely unknown and will be studied in this project. We have developed a fast and highly efficient CRISPR approach for gene tagging. With this in hand, we will tag endogenous NUPs with fluorophores such as NeonGreen and degron tags and study their behaviour, function and biogenesis in different cell lines and under various growth conditions.

The PhD student will be using a broad range of techniques such as biochemical approaches, conventional light and super resolution microscopy (STED; SIM) and electron microscopy for the analysis of the NPCs. CRISPR technology will be used for genomic tagging of human NUPs.
Firma Universität Heidelberg
69117 Heidelberg
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